An Experiment Showing the consequences of Polymerase Chain Response and Gel Electrophoresis on a DNA Intron

Abstract:

This experiment was made to show how Polymerase Chain Reaction (PCR) and Gel Electrophoresis can be utilised together to amplify a particular DNA intron- Alu sequence on the PV92 region of chromosome 16, via PCR and examine it via Gel Electrophoresis. Cheek cell samples from two college students were acquired, the DNA from the cells was purified and offered as template for the PCR. After 40 thermal cycles, the DNA location of curiosity was amplified and loaded into Gel Electrophoresis to get separated by segment size. The chromosomes that contained Alu sequence must have created a 941 bp band, and the chromosomes that didn't support the Alu sequence must have formed a 641 bp band in the gel when seen under UV light. Our gel electrophoresis results had been unsuccessful for the college student samples, however, they were powerful for the homozygous (+/+ and -/-) and heterozygous (+/-) control DNA samples.

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Introduction:

The human genome includes structural genes (which will be genes that code for proteins) and nonstructural genes (which will be genes that code for tRNA and rRNA). Every cell in our body provides the complete genome. However, different facets (like the located area of the cell and its environment) determine which genes will be being expressed in a particular